Targeting Grb2 SH3 Domains with Affimer Proteins Provides Novel Insights into Ras Signalling Modulation. AAS Tang, A Macdonald, MJ McPherson, DC Tomlinson. Biomolecules 14 (8), 1040
Affimer reagents enable targeted delivery of therapeutic agents and RNA via Virus-Like Particles. Roberts SE, Martin HL, Al-Qallaf D, Tang AA, Tiede C, Gaule TG, Dobon-Alonso A, Overman R, Shah S, Peyret H, Saunders K, Bon R, Manfield I, Bell SM, Lomonossoff GP, Speirs V, Tomlinson DC. iScience. 2024 July in press.
Exploiting the Affimer platform against influenza A virus. O Debski-Antoniak, A Flynn, DP Klebl, MH Rojas Rechy, C Tiede, IA Wilson, SP Muench, D Tomlinson, J Fontana. Mbio 15 (8), e01804-24
Affimer reagents as tool molecules to modulate platelet GPVI-ligand interactions and specifically bind GPVI dimer. Xu RG, Tiede C, Calabrese AN, Cheah LT, Adams TL, Gauer JS, Hindle MS, Webb BA, Yates DM, Slater A, Duval C, Naseem KM, Herr AB, Tomlinson DC, Watson SP, Ariëns RAS. Blood Adv. 2024 Jun in press
Photoactive imaging and therapy for colorectal cancer using a CEA-Affimer conjugated Foslip nanoparticle. Khaled YS, Khot MI, Aiyappa-Maudsley R, Maisey T, Pramanik A, Tiernan J, Lintern N, Al-Enezi E, Shamsuddin SH, Tomlinson D, Coletta L, Millner PA, Hughes TA, Jayne DG. Nanoscale. 2024 Apr 4;16(14):7185-7199. doi: 10.1039/d3nr04118b.
Therapeutic drug monitoring of immunotherapies with novel Affimer-NanoBiT sensor construct. Campbell E, Adamson H, Luxton T, Tiede C, Wälti C, Tomlinson DC, Jeuken LJC.Sens Diagn. 2023 Nov 1;3(1):104-111. doi: 10.1039/d3sd00126a. eCollection 2024 Jan 18.
"Affimer" synthetic protein scaffolds block oxidized LDL binding to the LOX-1 scavenger receptor and inhibit ERK1/2 activation. Roper BWR, Tiede C, Abdul-Zani I, Cuthbert GA, Jade D, Al-Aufi A, Critchley WR, Saikia Q, Homer-Vanniasinkam S, Sawamura T, McPherson MJ, Harrison MA, Tomlinson DC, Ponnambalam S.J Biol Chem. 2023 Nov;299(11):105325. doi: 10.1016/j.jbc.2023.105325. Epub 2023 Oct 5.
Affimer-mediated locking of p21-activated kinase 5 in an intermediate activation state results in kinase inhibition. Martin HL, Turner AL, Higgins J, Tang AA, Tiede C, Taylor T, Siripanthong S, Adams TL, Manfield IW, Bell SM, Morrison EE, Bond J, Trinh CH, Hurst CD, Knowles MA, Bayliss RW, Tomlinson DC. Cell Rep. 2023 Sep 29;42(10):113184. doi: 10.1016/j.celrep.2023.113184.
Enzyme - Switch sensors for therapeutic drug monitoring of immunotherapies. Campbell E, Adamson H, Kohl D, Tiede C, Wälti C, Tomlinson DC, Jeuken LJC. Biosens Bioelectron. 2023 Oct 1;237:115488. doi: 10.1016/j.bios.2023.115488. Epub 2023 Jun 30
Validation of a 3D perfused cell culture platform as a tool for humanised preclinical drug testing in breast cancer using established cell lines and patient-derived tissues. Liu P, Roberts S, Shoemaker JT, Vukasinovic J, Tomlinson DC, Speirs V.PLoS One. 2023 Mar 16;18(3):e0283044. doi: 10.1371/journal.pone.0283044.
Affimers targeting proteins in the cardiomyocyte Z-disc: Novel tools that improve imaging of heart tissue. Parker F, Tang AAS, Rogers B, Carrington G, Dos Remedios C, Li A, Tomlinson D, Peckham M. Front Cardiovasc Med. 2023 Feb 14;10:1094563. doi: 10.3389/fcvm.2023.1094563. eCollection 2023.
VelcroVax: a “Bolt-On” Vaccine Platform for Glycoprotein Display. Natalie J Kingston, Keith Grehan, Joseph S Snowden, Mark Hassall, Jehad Alzahrani, Guido C Paesen, Lee Sherry, Connor Hayward, Amy Roe, Sam Stephen, Darren Tomlinson, Antra Zeltina, Katie J Doores, Neil A Ranson, Martin Stacey, Mark Page, Nicola J Rose, Thomas A Bowden, David J Rowlands, Nicola J Stonehouse. Msphere, e00568-22, 2023
Fibrin-GPVI interaction increases platelet procoagulant activity and impacts clot structure. Julia S Gauer, Cédric Duval, Rui-Gang Xu, Fraser L Macrae, Helen R McPherson, Christian Tiede, Darren Tomlinson, Steve P Watson, Robert AS Ariëns. Journal of Thrombosis and Haemostasis, 2022
Affimers and nanobodies as molecular probes and their applications in imaging. Cordell P, Carrington G, Curd A, Parker F, Tomlinson D, Peckham M. J Cell Sci. 2022 Jul 15;135(14):jcs259168. doi: 10.1242/jcs.259168. Epub 2022 Jul 18. PMID: 35848463
Labeling of a mutant estrogen receptor with an Affimer in a breast cancer cell line. Ren P, Tiede C, Fanning SW, Adams T, Speirs V, Nelson ER, Cheng C, Moore TW, Greene GL, Tomlinson D, Selvin PR. Biophys J. 2022 Oct 4;121(19):3651-3662. doi: 10.1016/j.bpj.2022.06.028. Epub 2022 Jun 30.PMID: 35778844
Rapid Quantification of C. difficile Glutamate Dehydrogenase and Toxin B (TcdB) with a NanoBiT Split-Luciferase Assay. Adamson H, Ajayi MO, Gilroy KE, McPherson MJ, Tomlinson DC, Jeuken LJC. Anal Chem. 2022 Jun 14;94(23):8156-8163. doi: 10.1021/acs.analchem.1c05206. Epub 2022 May 28. PMID: 35634999 *
Protein-conjugated microbubbles for the selective targeting of S. aureus biofilms. Caudwell JA, Tinkler JM, Johnson BRG, McDowall KJ, Alsulaimani F, Tiede C, Tomlinson DC, Freear S, Turnbull WB, Evans SD, Sandoe JAT. Biofilm. 2022 Mar 19;4:100074. doi: 10.1016/j.bioflm.2022.100074. eCollection 2022 Dec.
Affinity purification of fibrinogen using an Affimer column. Pechlivani N, Kearney KJ, Tiede C, Cheah R, Phoenix F, Ponnambalam S, Ault JR, McPherson MJ, Tomlinson DC, Ajjan RA. Biochim Biophys Acta Gen Subj. 2022 May;1866(5):130115. doi: 10.1016/j.bbagen.2022.130115. Epub 2022 Feb 28.
Purification and Analysis of Circulating Lipid Particles. Roper BWR, Al-Sayejh B, Al-Aufi A, Cuthbert GA, Lacey K, Homer-Vanniasinkam S, Harrison MA, Tomlinson DC, Ajjan R, Ponnambalam S. Methods Mol Biol. 2022;2419:193-212. doi: 10.1007/978-1-0716-1924-7_12.
Piggybacking on the Cholera Toxin: Identification of a CTB-Binding Protein as an Approach for Targeted Delivery of Proteins to Motor Neurons. Balmforth MR, Haigh J, Kumar V, Dai W, Tiede C, Tomlinson DC, Deuchars J, Webb ME, Turnbull WB. Bioconjug Chem. 2021 Oct 20;32(10):2205-2212. doi: 10.1021/acs.bioconjchem.1c00373. Epub 2021 Sep 26.
Affimer Tagged Cubosomes: Targeting of Carcinoembryonic Antigen Expressing Colorectal Cancer Cells Using In Vitro and In Vivo Models. Pramanik A, Xu Z, Shamsuddin SH, Khaled YS, Ingram N, Maisey T, Tomlinson D, Coletta PL, Jayne D, Hughes TA, Tyler AII, Millner PA. ACS Appl Mater Interfaces. 2022 Mar 9;14(9):11078-11091. doi: 10.1021/acsami.1c21655. Epub 2022 Feb 23.
Ras inhibiting biologics identify and probe druggable pockets including an SII-a3 allosteric site. Haza KZ, Martin HL, Rao A, Turner AL, Saunders SE, Petersen B, Tiede C, Tipping K, Tang AA, Ajayi M, Taylor T, Harvey M, Fishwick KM, Adams TL, Gaule TG, Trinh CH, Johnson M, Breeze AL, Edwards TA, McPherson MJ, Tomlinson DC. Nature Communications. 2021 June 30.*
Characterization and applications of a Crimean-Congo hemorrhagic fever virus nucleoprotein-specific Affimer: Inhibitory effects in viral replication and development of colorimetric diagnostic tests. Álvarez-Rodríguez B, Tiede C, Hoste ACR, Surtees RA, Trinh CH, Slack GS, Chamberlain J, Hewson R, Fresco A, Sastre P, Tomlinson DC, Millner PA, Edwards TA, Barr JN. Plos Negleted Tropical Diseases. 2020 June 3.
Profiling cytotoxic microRNAs in pediatric and adult glioblastoma cells by high-content screening, identification, and validation of miR-1300. Boissinot M, King H, Adams M, Higgins J, Shaw G, Ward TA, Steele LP, Tams D, Morton R, Polson E, da Silva B, Droop A, Hayes JL, Martin H, Laslo P, Morrison E, Tomlinson DC, Wurdak H, Bond J, Lawler SE, Short SC.Oncogene. 2020 Jul;39(30):5292-5306. doi: 10.1038/s41388-020-1360-y. Epub 2020 Jun 17.
Exploiting nanobodies and Affimers for superresolution imaging in light microscopy. G Carrington, D Tomlinson, M Peckham. Molecular biology of the cell 30 (22), 2737-2740
Affimer-enzyme-inhibitor switch sensor for rapid wash-free assays of multimeric proteins. Hope Adamson, Modupe Ajayi, Emma Campbell, Erika Brachi, Christian Tiede, Anna Ah-San Tang, Thomas Lewis Adams, Robert Ford, Alex Davidson, Matt Johnson, Michael John McPherson, Darren Tomlinson, Lars JC Jeuken ACS sensors, 2019
Affimers as anti-idiotypic affinity reagents for pharmacokinetic analysis of biotherapeutics. Hope Adamson, Amanda Nicholl, Christian Tiede, Anna A Tang, Alex Davidson, Helen Curd, Alex Wignall, Robert Ford, James Nuttall, Michael J McPherson, Matt Johnson, Darren C Tomlinson BioTechniques
Affimer reagents as tools in diagnosing plant virus diseases. Hesketh EL, Tiede C, Adamson H, Adams TL, Byrne MJ, Meshcheriakova Y, Kruse I, McPherson MJ, Lomonossoff GP, Tomlinson DC, Ranson NA.. 2019 May 17;9(1):7524. doi: 10.1038/s41598-019-43945-6.
Control of conformation in α-helix mimicking aromatic oligoamide foldamers through interactions between adjacent side-chains. Arrata I, Grison CM, Coubrough HM, Prabhakaran P, Little MA, Tomlinson DC, Webb ME, Wilson AJ. Org Biomol Chem. 2019 Apr 10;17(15):3861-3867. doi: 10.1039/c9ob00123a.PMID:30938392
Affimer proteins as a tool to modulate fibrinolysis, stabilize the blood clot and reduce bleeding complications. Kearney KJ, Pechlivani N, King R, Tiede C, Phoenix F, Cheah R, Macrae FL, Simmons KJ, Manfield IW, Smith KA, Spurgeon BEJ, Naseem KM, Ariens RAS, McPherson MJ, Tomlinson DC, Ajjan RA. Blood. 2018 Dec 13. pii: blood-2018-06-856195. doi: 10.1182/blood-2018-06-856195.
FGFR3 mutation increases bladder tumourigenesis by suppressing acute inflammation. Foth M, Ismail NFB, Kung JSC, Tomlinson D, Knowles MA, Eriksson P, Sjödahl G, Salmond JM, Sansom OJ, Iwata T.J Pathol. 2018 Nov;246(3):331-343. doi: 10.1002/path.5143. Epub 2018 Sep 19.
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Site-Specific Labeling of Affimers for DNA-PAINT Microscopy. Schlichthaerle T, Eklund AS, Schueder F, Strauss MT, Tiede C, Curd A, Ries J, Peckham M, Tomlinson DC, Jungmann R. Angew Chem Int Ed Engl. 2018 Jun 6. doi: 10.1002/anie.201804020.
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Affimer proteins for F-actin: novel affinity reagents that label F-actin in liveand fixed cells. Lopata A, Hughes R, Tiede C, Heissler SM, Sellers JR, Knight PJ, , Peckham M. Sci Rep. 2018; 8: 6572
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Sensitive and selective Affimer-functionalised interdigitated electrode-based capacitive biosensor for Her4 protein tumour biomarker detection. Zhurauski P, Arya SK, Jolly P, Tiede C, Tomlinson DC, Ferrigno PK, Estrela P. Biosensor and Bioelectronics. 108 (2018) 1-8
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Non-immunoglobulin scaffold proteins: Precision tools for studying protein-protein interactions in cancer. Martin HL, Bedford R, Heseltine SJ, Tang AA, Haza KZ, Rao A, McPherson MJ, Tomlinson DC*. New Biotechnology. Feb 20. pii: S1871-6784(17)30541-1. doi: 10.1016/j.nbt.2018.02.008
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Affimers inhibit immune complex binding to FcgRIIIa with high specificity through competitive and allosteric modes of action. Robinson JI, Baxter E, Owen RL, Thomsen M, Tomlinson DC, Waterhouse MP, Win SJ, Nettleship JE, Tiede C, Foster RJ, Owens RJ, Fishwick CWG, Harris SA, Goldman A, McPherson MJ, Morgan AW. Proc Natl Acad Sci U S A. 2017 Dec 15. pii: 201707856. doi: 10.1073/pnas.1707856115
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Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection. Tang AS, Tiede C, Hughes DJ, McPherson M, Tomlinson DC. Sci. Signal. 14 Nov 2017: Vol. 10, Issue 505, eaan0868 DOI: 10.1126/scisignal.aan0868
Generation of specific inhibitors of SUMO-1– and SUMO-2/3–mediated protein-protein interactions using Affimer (Adhiron) technology. Hughes DJ, Tiede C, Penswick N, Tang AS, Trinh CH, Mandal U, Zaja KZ, Gaule T, Howell G, Edwards TA, Duan J, Feyfant E, McPherson MJ, Tomlinson DC, Whitehouse A. Sci. Signal. 14 Nov 2017:Vol. 10, Issue 505, eaaj2005. DOI: 10.1126/scisignal.aaj2005
Development of an Affimer-antibody combined immunological diagnostic kit for glypican-3. Xie C, Tiede C, Zhang X, Wang C, Li Z, Xu X, McPherson MJ, Tomlinson DC, Xu W. Scientific Reports 7, Article number: 9608 (2017), doi:10.1038/s41598-017-10083-w
Ubiquitination of basal VEGFR2 regulates signal transduction and endothelial function. Smith GA, Fearnley GW, Abdul-Zani I, Wheatcroft SB, Tomlinson DC, Harrison MA, Ponnambalam S. Biol Open. 2017 Oct 15;6(10):1404-1415. doi: 10.1242/bio.027896.
Alternative reagents to antibodies in imaging applications. Bedford R, Tiede C, Hughes R, Curd A, McPherson MJ, Peckham M, Tomlinson DC. Biophys Rev. 2017 Jul 27. doi: 10.1007/s12551-017-0278-2. [Epub ahead of print] Review. PMID:28752365
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Affimer proteins are versatile and renewable affinity reagents.Tiede C, Bedford R, Heseltine SJ, Smith G, Wijetunga I, Ross R, AlQallaf D, Roberts AP, Balls A, Curd A, Hughes RE, Martin H, Needham SR, Zanetti-Domingues LC, Sadigh Y, Peacock TP, Tang AA, Gibson N, Kyle H, Platt GW, Ingram N, Taylor T, Coletta LP, Manfield I, Knowles M, Bell S, Esteves F, Maqbool A, Prasad RK, Drinkhill M, Bon RS, Patel V, Goodchild SA, Martin-Fernandez M, Owens RJ, Nettleship JE, Webb ME, Harrison M, Lippiat JD, Ponnambalam S, Peckham M, Smith A, Ferrigno PK, Johnson M, McPherson MJ, Tomlinson DC. Elife. 2017 Jun 27;6. pii: e24903. doi: 10.7554/eLife.24903.
Interfacing native and non-native peptides: using Affimers to recognise α-helix mimicking foldamers. Arrata I, Barnard A, Tomlinson DC, Wilson AJ. Chem Commun (Camb). 2017 Feb 20. doi: 10.1039/c6cc09395g.
Antibody Mimetics for the Detection of Small Organic Compounds Using a Quartz Crystal Microbalance.Koutsoumpeli E, Tiede C, Murray J, Tang A, Bon RS, Tomlinson DC, Johnson S.
Anal Chem. 2017 Feb 15. doi: 10.1021/acs.analchem.6b04790.
Label-free electrochemical impedance biosensor to detect human interleukin-8 in serum with sub-pg/ml sensitivity. Sharma R, Deacon SE, Nowak D, George SE, Szymonik MP, Tang AA, Tomlinson DC, Davies AG, McPherson MJ, Wälti C. Biosens Bioelectron. 2016 Jun 15;80:607-13. doi: 10.1016/j.bios.2016.02.028. Epub 2016 Feb 11.
Tumor Heterogeneity of Fibroblast Growth Factor Receptor 3 (FGFR3) Mutations in Invasive Bladder Cancer: Implications for Peri-Operative anti-FGFR3 Treatment. Pouessel D, Neuzillet Y, Mertens LS, van der Heijden MS, de Jong J, Sanders J, Peters D, Leroy K, Manceau A, Maille P, Soyeux P, Moktefi A, Semprez F, Vordos D, de la Taille A, Hurst CD, Tomlinson DC, Harnden P, Bostrom PJ, Mirtti T, Horenblas S, Loriot Y, Houédé N, Chevreau C, Beuzeboc P, Shariat SF, Sagalowsky AI, Ashfaq R, Burger M, Jewett MA, Zlotta AR, Broeks A, Bapat B, Knowles MA, Lotan Y, van der Kwast TH, Culine S, Allory Y, van Rhijn BW. Ann Oncol. 2016 Apr 18. pii: mdw170. [Epub ahead of print]
VEGF-A isoforms program differential VEGFR2 signal transduction, trafficking and proteolysis. Fearnley GW, Smith GA, Abdul-Zani I, Yuldasheva N, Mughal NA, Homer-Vanniasinkam S, Kearney MT, Zachary IC, Tomlinson DC, Harrison MA, Wheatcroft SB, Ponnambalam S. Biol Open. 2016 Apr 3. pii: bio.017434. doi: 10.1242/bio.017434.
VEGFR2 Trafficking, Signaling and Proteolysis is Regulated by the Ubiquitin Isopeptidase USP8. Smith GA, Fearnley GW, Abdul-Zani I, Wheatcroft SB, Tomlinson DC, Harrison MA, Ponnambalam S. Traffic. 2016 Jan;17(1):53-65. doi: 10.1111/tra.12341. Epub 2015 Dec 2.
Trivalent Gd-DOTA reagents for modification of proteins. Fisher MJ, Williamson DJ, Burslem GM, Plante JP, Manfield I, Tiede C, Ault JR, Stockley P, Plein S, Maqbool A, Tomlinson DC, Foster R, Warriner S, Bon RS. RSC Advances , 5 ( 116 ) pp. 96194 - 96200.
VEGFR2 signal transduction, trafficking and proteolysis is regulated by the ubiquitin isopeptidase USP8. Smith GA, Fearnley GW, Zani, IA, Wheatcroft SB, Tomlinson DC, Harrison MA, Ponnambalam S (2015) Traffic, In press.
Receptor tyrosine kinase structure and function in health and disease. Karpov O, Fearnley GW, Smith GA, Kankanala J, McPherson MJ, Tomlinson DC, Harrison MA, Ponnambalam S (2015) AIMS Biophysics 2:476-502.
The cellular response to vascular endothelial growth factors requires co-ordinated signal transduction, trafficking and proteolysis.Smith GA, Fearnley GW, Tomlinson DC, Harrison MA, Ponnambalam S.
Biosci Rep. 2015 Aug 18. pii: BSR20150171. [Epub ahead of print] Review.
Phage Display Selected Magnetite Interacting Adhirons For Shape Controlled Nanoparticle Synthesis. A E Rawlings, J P Bramble, A A S Tang, L A Somner, Amy E Monnington, D J Cooke, M J McPherson, D C Tomlinson, S S Staniland.. Chemical Science 2015 Advanced article
An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes. G Wheway, M Schmidts, D Mans, K Szymanska, T Nguyen, H Racher, I G Phelps, G Toedt, J Kennedy, K Wunderlich, N Sorusch, Z Abdelhamed, S Natarajan, W Herridge, J van Reeuwijk, N Horn, K Boldt, D Parry, S J.F. Letteboer, S Roosing, M Adams, S M Bell, J Bond, J Higgins, E E Morrison, D Tomlinson, G G Slaats, T J P van Dam, L Huang, K Kessler, A Gießl, C Logan, E Boyle, J Shendure, S Anazi, M Aldahmesh, S Al Hazzaa, R Hegele, C Ober, P Frosk, A A Mhanni, B N Chodirker, A E Chudley, R Lamont, F P Bernier, C L Beaulieu, P Gordon, R T Pon, C Donahue, A J Barkovich, L Wolf, C Toomes, C Thiel, K M Boycott, M McKibbin, C Inglehearn, UK10K Consortium, University of Washington Center for Mendelian Genomics, F Stewart, H Omran, M A Huynen, P I Sergouniotis, F Alkuraya, J S Parboosingh, M Innes, C Willoughby, R Giles, A Webster, M Ueffing, O Blacque, J Gleeson, U Wolfrum, P Beales, T Gibson, D Doherty, H Mitchison, R Roepman, and C Johnson. Nat Cell Biol. 2015 Aug;17(8):1074-87.
Exploration of the HIF-1α/p300 binding interface using peptide and Adhiron phage display technologies to locate binding hot-spots for inhibitor development. H. F. Kyle, K. F. Wickson, J. Stott, G. M. Burslem, A. L. Breeze, C. Tiede , D. C. Tomlinson, S. L. Warriner, A. Nelson, A. J. Wilson and T. A. Edwards. Mol Biosyst. 2015 Jul 2. [Epub ahead of print]
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Development and characterization of a 3D multi-cellular in vitro model of normal human breast: a tool for cancer initiation studies. Nash CE, Mavria G, Baxter EW, Holliday Dl, Tomlinson DC, Treanor D, Novitskaya V, Berditchevski F, Hanby AM and Speirs V. Oncotarget. 2015 May 30;6(15):13731-41.
Vascular endothelial growth factor and receptor function in health, metabolism and disease. Smith GA, Fearnley GW, Wheatcroft SB, Harrison MA, Tomlinson DC, Ponnambalam S. J Inherit Metab Dis. 2015 Jul;38(4):753-63.
Selective and potent proteomimetic inhibitors of intracellular protein-protein interactions. A Barnard, Kérya Long, H L. Martin, J A. Miles, Thomas A. Edwards, D C. Tomlinson, A Macdonald, A J. Wilson. Angew Chem Int Ed Engl. 2015 Mar 2;54(10):2960-5.
Antibody mimetic receptor proteins for label-free biosensors. Raina, M., Sharma, R., Deacon, S.E., Tiede, C., Tomlinson, D., Davies, A.G., McPherson, M.J., Walti, C., 2015. Analyst 140, 803–810.
Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications. C Tiede, A Tang, S Deacon, U Mandal, J Nettleship, D Harrison, R Owen, R Owens, S George, D Tomlinson, M McPherson. Protein Eng Des Sel. 2014 May;27(5):145-55.
High-content, high-throughput screening for the identification of cytotoxic compounds based on cell morphology and cell proliferation markers. H L Martin, M Adams, J Higgins, J Bond, E E Morrison, S M Bell, S Warriner, A Nelson and D C Tomlinson. PloS One. 2014 Feb 5;9(2):e88338.
Multidrug resistant breast cancer: current perspectives. H L Martin, L Smith and D C Tomlinson. Breast Cancer: Targets and Therapy. 2014;2014:6;1-6
The use of a neutral peptide aptamer scaffold to anchor BH3 peptides constitutes a viable approach to studying their function. D Tomlinson, L Stadler, T Lee, M Knowles, P Ko Ferrigno. Cell Death and Differentiation (2014) 5, e1037; doi:10.1038/cddis.2013.564
Proof of concept study to identify candidate biomarkers of fibrosis using high throughput peptide aptamer microarray and validate by enzyme linked immunosorbant assay. S Straw, P Ko Ferrigno, Q Song, D Tomlinson, F Del Galdo. Journal of Biomedical Science and Engineering. 2013 Vol.6 No.8B
Vascular endothelial growth factor A-stimulated signaling from endosomes in primary endothelial cells. Fearnley G, Smith GA, Odell AF, Latham AM, Wheatcroft SB, Harrison MA, Tomlinson DC, Ponnambalam S (2014) Meth Enzymol 535:265-292.
Vascular endothelial growth factor-A regulation of blood vessel sprouting in health and disease. Fearnley GW, Smith GA, Harrison MA, Wheatcroft SB, Tomlinson DC, Ponnambalam S. OA Biochemistry 2013 Feb 01;1(1):5.
A High-Throughput Assay to Identify Modifiers of Premature Chromosome Condensation. Adams M, Cookson VJ, Higgins J, Martin HL, Tomlinson DC, Bond J, Morrison EE, Bell SM.
J Biomol Screen. 2013 Aug 27.
A Decade of FGF Receptor Research in Bladder Cancer: Past, Present, and Future Challenges. di Martino E, Tomlinson DC, Knowles MA. Adv Urol. 2012;2012:429213.
FGFR1-Induced Epithelial to Mesenchymal Transition through MAPK/PLCγ/COX-2-Mediated Mechanisms. Tomlinson DC, Baxter EW, Loadman PM, Hull MA, Knowles MA. PloS One. 2012;7(6):e38972. Epub 2012 Jun 12.
Mechanisms of FGFR3 actions in endocrine resistant breast cancer, Tomlinson DC, Knowles MA, Speirs V. Int J Cancer. 2012 Jun 15;130(12):2857-66
Structure-function studies of an engineered scaffold protein derived from Stefin A. II: Development and Applications of the SQT variant. Stadler L, Hoffmann T, Tomlinson D, Song Q, Lee T, Busby M, Nyathi Y, Gendra E, Tiede C, Flanagan K, Cockell S, Wipat A, Harwood C, Wagner S, Knowles M, Davis J, Ko Ferrigno P.Protein Eng Des Sel. 2011 Sep;24(9):751-63
Small molecule FGF receptor FGFR-dependent urothelial carcinoma growth in vitro and in vivo. inhibitor Lamont FR, Tomlinson DC, Cooper PA, Shnyder SD, Chester JD, Knowles MA. Br J Cancer. 2011 Jan 4;104(1):75-82. Epub 2010 Nov 30
Altered splicing of FGFR1 is associated with high tumour grade and stage and leads to increased sensitivity to FGF1 in bladder cancer. Tomlinson DC and Knowles MA, Am J Path, 2010 Nov;177(5):2379-86.
FGFR3 mutation affects cell growth, apoptosis and attachment in keratinocytes. Hafner C, Di Martino E, Pitt E, Stempfl T, Tomlinson D, Hartmann A, Landthaler M, Knowles M, Vogt T.Exp Cell Res. 2010 Jul 15;316(12):2008-16.
Mutant fibroblast growth factor receptor 3 induces intracellular signaling and cellular transformation in a cell type- and mutation-specific manner. di Martino E, L'Hôte CG, Kennedy W, Tomlinson DC, Knowles MA. Oncogene. 2009 Dec 3;28(48):4306-16.
Fibroblast growth factor receptor 1 promotes proliferation and survival via activation of the mitogen-activated protein kinase pathway in bladder cancer. Tomlinson DC, Lamont FR, Shnyder SD, Knowles MA. Cancer Res. 2009 Jun 1;69(11):4613-20.
Inactivation of the Rb pathway and overexpression of both isoforms of E2F3 are obligate events in bladder tumours with 6p22 amplification. Hurst CD, Tomlinson DC, Williams SV, Platt FM, Knowles MA. Oncogene. 2008 Apr 24;27(19):2716-27.
FGFR3 protein expression and its relationship to mutation status and prognostic variables in bladder cancer. Tomlinson DC, Baldo O, Harnden P, Knowles MA. J Pathol. 2007 Sep;213(1):91-8
Knockdown by shRNA identifies S249C mutant FGFR3 as a potential therapeutic target in bladder cancer. Tomlinson DC, Hurst CD, Knowles MA. Oncogene. 2007 Aug 30;26(40):5889-99.
Alternative splicing of fibroblast growth factor receptor 3 produces a secreted isoform that inhibits fibroblast growth factor-induced proliferation and is repressed in urothelial carcinoma cell lines. Tomlinson DC, L'Hote CG, Kennedy W, Pitt E, Knowles MA. Cancer Research 2005;65(22):10441-9.
FGFR3 and Ras gene mutations are mutually exclusive genetic events in urothelial cell carcinoma. Jebar AH, Hurst CD, Tomlinson DC, Johnston C, Taylor CF, Knowles MA. Oncogene 2005;24(33):5218-25.
Differential effects of transforming growth factor-beta1 on cellular proliferation in the developing prostate. Tomlinson DC, Freestone SH, Grace OC, Thomson AA. Endocrinology. 2004 Sep;145(9):4292-300. Epub 2004 Jun 10.
Regulation of Fgf10 gene expression in the prostate: identification of transforming growth factor-beta1 and promoter elements. Tomlinson DC, Grindley JC, Thomson AA. Endocrinology. 2004 Apr;145(4):1988-95. Epub 2004 Jan 15.
Sonic hedgehog regulates prostatic growth and epithelial differentiation. Freestone SH, Marker P, Grace OC, Tomlinson DC, Cunha GR, Harnden P, Thomson AA. Dev Biol. 2003 Dec 15;264(2):352-62.
