Exploiting nanobodies and Affimers for superresolution imaging in light microscopy. G Carrington, D Tomlinson, M Peckham. Molecular biology of the cell 30 (22), 2737-2740

 

Affimer-enzyme-inhibitor switch sensor for rapid wash-free assays of multimeric proteins. Hope Adamson, Modupe Ajayi, Emma Campbell, Erika Brachi, Christian Tiede, Anna Ah-San Tang, Thomas Lewis Adams, Robert Ford, Alex Davidson, Matt Johnson, Michael John McPherson, Darren Tomlinson, Lars JC Jeuken ACS sensors, 2019

 

Affimers as anti-idiotypic affinity reagents for pharmacokinetic analysis of biotherapeutics. Hope Adamson, Amanda Nicholl, Christian Tiede, Anna A Tang, Alex Davidson, Helen Curd, Alex Wignall, Robert Ford, James Nuttall, Michael J McPherson, Matt Johnson, Darren C Tomlinson BioTechniques

 

Affimer reagents as tools in diagnosing plant virus diseases. Hesketh EL, Tiede C, Adamson H, Adams TL, Byrne MJ, Meshcheriakova Y, Kruse I, McPherson MJ, Lomonossoff GP, Tomlinson DC, Ranson NA.. 2019 May 17;9(1):7524. doi: 10.1038/s41598-019-43945-6.

 

Control of conformation in α-helix mimicking aromatic oligoamide foldamers through interactions between adjacent side-chains. Arrata I, Grison CM, Coubrough HM, Prabhakaran P, Little MA, Tomlinson DC, Webb ME, Wilson AJ. Org Biomol Chem. 2019 Apr 10;17(15):3861-3867. doi: 10.1039/c9ob00123a.PMID:30938392

 

Affimer proteins as a tool to modulate fibrinolysis, stabilize the blood clot and reduce bleeding complications. Kearney KJ, Pechlivani N, King R, Tiede C, Phoenix F, Cheah R, Macrae FL, Simmons KJ, Manfield IW, Smith KA, Spurgeon BEJ, Naseem KM, Ariens RAS, McPherson MJ, Tomlinson DC, Ajjan RA. Blood. 2018 Dec 13. pii: blood-2018-06-856195. doi: 10.1182/blood-2018-06-856195.

 

FGFR3 mutation increases bladder tumourigenesis by suppressing acute inflammation. Foth M, Ismail NFB, Kung JSC, Tomlinson D, Knowles MA, Eriksson P, Sjödahl G, Salmond JM, Sansom OJ, Iwata T.J Pathol. 2018 Nov;246(3):331-343. doi: 10.1002/path.5143. Epub 2018 Sep 19.

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Site-Specific Labeling of Affimers for DNA-PAINT Microscopy. Schlichthaerle T, Eklund AS, Schueder F, Strauss MT, Tiede C, Curd A, Ries J, Peckham M, Tomlinson DC, Jungmann R. Angew Chem Int Ed Engl. 2018 Jun 6. doi: 10.1002/anie.201804020.

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Affimer proteins for F-actin: novel affinity reagents that label F-actin in liveand fixed cells. Lopata A, Hughes R, Tiede C, Heissler SM, Sellers JR, Knight PJ, , Peckham M. Sci Rep. 2018; 8: 6572

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Sensitive and selective Affimer-functionalised interdigitated electrode-based capacitive biosensor for Her4 protein tumour biomarker detection. Zhurauski P, Arya SK, Jolly P, Tiede C, Tomlinson DC, Ferrigno PK, Estrela P. Biosensor and Bioelectronics. 108 (2018) 1-8

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Non-immunoglobulin scaffold proteins: Precision tools for studying protein-protein interactions in cancer. Martin HL, Bedford R, Heseltine SJ, Tang AA, Haza KZ, Rao A, McPherson MJ, Tomlinson DC*. New Biotechnology. Feb 20. pii: S1871-6784(17)30541-1. doi: 10.1016/j.nbt.2018.02.008

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Affimers inhibit immune complex binding to FcgRIIIa with high specificity through competitive and allosteric modes of action. Robinson JI, Baxter E, Owen RL, Thomsen M, Tomlinson DC, Waterhouse MP, Win SJ, Nettleship JE, Tiede C, Foster RJ, Owens RJ, Fishwick CWG, Harris SA, Goldman A, McPherson MJ, Morgan AW. Proc Natl Acad Sci U S A. 2017 Dec 15. pii: 201707856. doi: 10.1073/pnas.1707856115

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Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection. Tang AS, Tiede C, Hughes DJ, McPherson M, Tomlinson DC. Sci. Signal.  14 Nov 2017: Vol. 10, Issue 505, eaan0868 DOI: 10.1126/scisignal.aan0868

 

Generation of specific inhibitors of SUMO-1– and SUMO-2/3–mediated protein-protein interactions using Affimer (Adhiron) technology. Hughes DJ, Tiede C, Penswick N, Tang AS, Trinh CH, Mandal U, Zaja KZ, Gaule T, Howell G, Edwards TA, Duan J, Feyfant E, McPherson MJ, Tomlinson DC, Whitehouse A. Sci. Signal.  14 Nov 2017:Vol. 10, Issue 505, eaaj2005. DOI: 10.1126/scisignal.aaj2005

 

Development of an Affimer-antibody combined immunological diagnostic kit for glypican-3. Xie C, Tiede C, Zhang X, Wang C, Li Z, Xu X, McPherson MJ, Tomlinson DC, Xu W.  Scientific Reports 7, Article number: 9608 (2017), doi:10.1038/s41598-017-10083-w

 

Ubiquitination of basal VEGFR2 regulates signal transduction and endothelial function. Smith GA, Fearnley GW, Abdul-Zani I, Wheatcroft SB, Tomlinson DC, Harrison MA, Ponnambalam S. Biol Open. 2017 Oct 15;6(10):1404-1415. doi: 10.1242/bio.027896.

 

Alternative reagents to antibodies in imaging applications. Bedford R, Tiede C, Hughes R, Curd A, McPherson MJ, Peckham M, Tomlinson DC. Biophys Rev. 2017 Jul 27. doi: 10.1007/s12551-017-0278-2. [Epub ahead of print] Review. PMID:28752365

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Affimer proteins are versatile and renewable affinity reagents.Tiede C, Bedford R, Heseltine SJ, Smith G, Wijetunga I, Ross R, AlQallaf D, Roberts AP, Balls A, Curd A, Hughes RE, Martin H, Needham SR, Zanetti-Domingues LC, Sadigh Y, Peacock TP, Tang AA, Gibson N, Kyle H, Platt GW, Ingram N, Taylor T, Coletta LP, Manfield I, Knowles M, Bell S, Esteves F, Maqbool A, Prasad RK, Drinkhill M, Bon RS, Patel V, Goodchild SA, Martin-Fernandez M, Owens RJ, Nettleship JE, Webb ME, Harrison M, Lippiat JD, Ponnambalam S, Peckham M, Smith A, Ferrigno PK, Johnson M, McPherson MJ, Tomlinson DC. Elife. 2017 Jun 27;6. pii: e24903. doi: 10.7554/eLife.24903.

 

Interfacing native and non-native peptides: using Affimers to recognise α-helix mimicking foldamers. Arrata I, Barnard A, Tomlinson DC, Wilson AJ. Chem Commun (Camb). 2017 Feb 20. doi: 10.1039/c6cc09395g.

 

Antibody Mimetics for the Detection of Small Organic Compounds Using a Quartz Crystal Microbalance.Koutsoumpeli E, Tiede C, Murray J, Tang A, Bon RS, Tomlinson DC, Johnson S.

Anal Chem. 2017 Feb 15. doi: 10.1021/acs.analchem.6b04790.

 

 

Label-free electrochemical impedance biosensor to detect human interleukin-8 in serum with sub-pg/ml sensitivity. Sharma R, Deacon SE, Nowak D, George SE, Szymonik MP, Tang AA, Tomlinson DC, Davies AG, McPherson MJ, Wälti C. Biosens Bioelectron. 2016 Jun 15;80:607-13. doi: 10.1016/j.bios.2016.02.028. Epub 2016 Feb 11.

 

Tumor Heterogeneity of Fibroblast Growth Factor Receptor 3 (FGFR3) Mutations in Invasive Bladder Cancer: Implications for Peri-Operative anti-FGFR3 Treatment. Pouessel D, Neuzillet Y, Mertens LS, van der Heijden MS, de Jong J, Sanders J, Peters D, Leroy K, Manceau A, Maille P, Soyeux P, Moktefi A, Semprez F, Vordos D, de la Taille A, Hurst CD, Tomlinson DC, Harnden P, Bostrom PJ, Mirtti T, Horenblas S, Loriot Y, Houédé N, Chevreau C, Beuzeboc P, Shariat SF, Sagalowsky AI, Ashfaq R, Burger M, Jewett MA, Zlotta AR, Broeks A, Bapat B, Knowles MA, Lotan Y, van der Kwast TH, Culine S, Allory Y, van Rhijn BW. Ann Oncol. 2016 Apr 18. pii: mdw170. [Epub ahead of print]

 

VEGF-A isoforms program differential VEGFR2 signal transduction, trafficking and proteolysis. Fearnley GW, Smith GA, Abdul-Zani I, Yuldasheva N, Mughal NA, Homer-Vanniasinkam S, Kearney MT, Zachary IC, Tomlinson DC, Harrison MA, Wheatcroft SB, Ponnambalam S. Biol Open. 2016 Apr 3. pii: bio.017434. doi: 10.1242/bio.017434.

 

VEGFR2 Trafficking, Signaling and Proteolysis is Regulated by the Ubiquitin Isopeptidase USP8. Smith GA, Fearnley GW, Abdul-Zani I, Wheatcroft SB, Tomlinson DC, Harrison MA, Ponnambalam S. Traffic. 2016 Jan;17(1):53-65. doi: 10.1111/tra.12341. Epub 2015 Dec 2.

 

Trivalent Gd-DOTA reagents for modification of proteins. Fisher MJ, Williamson DJ, Burslem GM, Plante JP, Manfield I, Tiede C, Ault JR, Stockley P, Plein S, Maqbool A, Tomlinson DC,  Foster R, Warriner S, Bon RS. RSC Advances , 5 ( 116 ) pp. 96194 - 96200.

 

VEGFR2 signal transduction, trafficking and proteolysis is regulated by the ubiquitin isopeptidase USP8. Smith GA, Fearnley GW, Zani, IA, Wheatcroft SB, Tomlinson DC, Harrison MA, Ponnambalam S (2015) Traffic, In press.

 

Receptor tyrosine kinase structure and function in health and disease. Karpov O, Fearnley GW, Smith GA, Kankanala J, McPherson MJ, Tomlinson DC, Harrison MA, Ponnambalam S (2015) AIMS Biophysics 2:476-502.

 

The cellular response to vascular endothelial growth factors requires co-ordinated signal transduction, trafficking and proteolysis.Smith GA, Fearnley GW, Tomlinson DC, Harrison MA, Ponnambalam S.

Biosci Rep. 2015 Aug 18. pii: BSR20150171. [Epub ahead of print] Review.

 

Phage Display Selected Magnetite Interacting Adhirons For Shape Controlled Nanoparticle Synthesis. A E Rawlings, J P Bramble, A A S Tang, L A Somner, Amy E Monnington, D J Cooke, M J McPherson, D C Tomlinson, S S Staniland.. Chemical Science 2015 Advanced article

 

An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes. G Wheway, M Schmidts, D Mans, K Szymanska, T Nguyen, H Racher, I G Phelps, G Toedt, J Kennedy, K Wunderlich, N Sorusch, Z Abdelhamed, S Natarajan, W Herridge, J van Reeuwijk, N Horn, K Boldt, D Parry, S J.F. Letteboer, S Roosing, M Adams, S M Bell, J Bond, J Higgins, E E Morrison, D Tomlinson, G G Slaats, T J P van Dam, L Huang, K Kessler, A Gießl, C Logan, E Boyle, J Shendure, S Anazi, M Aldahmesh, S Al Hazzaa, R Hegele, C Ober, P Frosk, A A Mhanni, B N Chodirker, A E Chudley, R Lamont, F P Bernier, C L Beaulieu, P Gordon, R T Pon, C Donahue, A J Barkovich, L Wolf, C Toomes, C Thiel, K M Boycott, M McKibbin, C Inglehearn, UK10K Consortium, University of Washington Center for Mendelian Genomics, F Stewart, H Omran, M A Huynen, P I Sergouniotis, F Alkuraya, J S Parboosingh, M Innes, C Willoughby, R Giles, A Webster, M Ueffing, O Blacque, J Gleeson, U Wolfrum, P Beales, T Gibson, D Doherty, H Mitchison, R Roepman, and C Johnson. Nat Cell Biol. 2015 Aug;17(8):1074-87.

 

Exploration of the HIF-1α/p300 binding interface using peptide and Adhiron phage display technologies to locate binding hot-spots for inhibitor development. H. F. Kyle, K. F. Wickson, J. Stott, G. M. Burslem, A. L. Breeze, C. Tiede ,  D. C. Tomlinson, S. L. Warriner, A. Nelson, A. J. Wilson and T. A. Edwards. Mol Biosyst. 2015 Jul 2. [Epub ahead of print]

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Development and characterization of a 3D multi-cellular in vitro model of normal human breast: a tool for cancer initiation studies. Nash CE, Mavria G, Baxter EW, Holliday Dl, Tomlinson DC, Treanor D, Novitskaya V, Berditchevski F, Hanby AM and Speirs V. Oncotarget. 2015 May 30;6(15):13731-41.

 

Vascular endothelial growth factor and receptor function in health, metabolism and disease. Smith GA, Fearnley GW, Wheatcroft SB, Harrison MA, Tomlinson DC, Ponnambalam S. J Inherit Metab Dis. 2015 Jul;38(4):753-63.

 

Selective and potent proteomimetic inhibitors of intracellular protein-protein interactions. A Barnard, Kérya Long,  H L. Martin, J A. Miles, Thomas A. Edwards,  D C. Tomlinson, A Macdonald, A J. Wilson. Angew Chem Int Ed Engl. 2015 Mar 2;54(10):2960-5.

 

Antibody mimetic receptor proteins for label-free biosensors. Raina, M., Sharma, R., Deacon, S.E., Tiede, C., Tomlinson, D., Davies, A.G., McPherson, M.J., Walti, C., 2015. Analyst 140, 803–810.

 

Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications. C Tiede, A Tang, S Deacon, U Mandal, J Nettleship, D Harrison, R Owen, R Owens, S George, D Tomlinson, M McPherson. Protein Eng Des Sel. 2014 May;27(5):145-55.

 

High-content, high-throughput screening for the identification of cytotoxic compounds based on cell morphology and cell proliferation markers. H L Martin, M Adams, J Higgins, J Bond, E E Morrison, S M Bell, S Warriner, A Nelson and  D C Tomlinson. PloS One. 2014 Feb 5;9(2):e88338.

 

Multidrug resistant breast cancer: current perspectives. H L Martin, L Smith and D C Tomlinson. Breast Cancer: Targets and Therapy.  2014;2014:6;1-6

 

The use of a neutral peptide aptamer scaffold to anchor BH3 peptides constitutes a viable approach to studying their function. D Tomlinson, L Stadler, T Lee, M Knowles, P Ko Ferrigno. Cell Death and Differentiation (2014) 5, e1037; doi:10.1038/cddis.2013.564

 

Proof of concept study to identify candidate biomarkers of fibrosis using high throughput peptide aptamer microarray and validate by enzyme linked immunosorbant assay. S Straw, P Ko Ferrigno, Q Song, D Tomlinson, F Del Galdo. Journal of Biomedical Science and Engineering. 2013 Vol.6 No.8B

 

Vascular endothelial growth factor A-stimulated signaling from endosomes in primary endothelial cells. Fearnley G, Smith GA, Odell AF, Latham AM, Wheatcroft SB, Harrison MA, Tomlinson DC,  Ponnambalam S (2014) Meth Enzymol 535:265-292.

 

Vascular endothelial growth factor-A regulation of blood vessel sprouting in health and disease. Fearnley GW, Smith GA, Harrison MA, Wheatcroft SB, Tomlinson DC, Ponnambalam S. OA Biochemistry 2013 Feb 01;1(1):5.

 

A High-Throughput Assay to Identify Modifiers of Premature Chromosome Condensation. Adams M, Cookson VJ, Higgins J, Martin HL, Tomlinson DC, Bond J, Morrison EE, Bell SM.

J Biomol Screen. 2013 Aug 27.

 

A Decade of FGF Receptor Research in Bladder Cancer: Past, Present, and Future Challenges. di Martino E, Tomlinson DC, Knowles MA. Adv Urol. 2012;2012:429213.

 

FGFR1-Induced Epithelial to Mesenchymal Transition through MAPK/PLCγ/COX-2-Mediated Mechanisms. Tomlinson DC, Baxter EW, Loadman PM, Hull MA, Knowles MA. PloS One. 2012;7(6):e38972. Epub 2012 Jun 12.

 

Mechanisms of FGFR3 actions in endocrine resistant breast cancer, Tomlinson DC, Knowles MA, Speirs V. Int J Cancer. 2012 Jun 15;130(12):2857-66

 

Structure-function studies of an engineered scaffold protein derived from Stefin A. II: Development and Applications of the SQT variant. Stadler L, Hoffmann T, Tomlinson D, Song Q, Lee T, Busby M, Nyathi Y, Gendra E, Tiede C, Flanagan K, Cockell S, Wipat A, Harwood C, Wagner S, Knowles M, Davis J, Ko Ferrigno P.Protein Eng Des Sel. 2011 Sep;24(9):751-63

 

Small molecule FGF receptor FGFR-dependent urothelial carcinoma growth in vitro and in vivo. inhibitor Lamont FR, Tomlinson DC, Cooper PA, Shnyder SD, Chester JD, Knowles MA. Br J Cancer. 2011 Jan 4;104(1):75-82. Epub 2010 Nov 30

 

Altered splicing of FGFR1 is associated with high tumour grade and stage and leads to increased sensitivity to FGF1 in bladder cancer. Tomlinson DC and Knowles MA, Am J Path, 2010 Nov;177(5):2379-86.

 

FGFR3 mutation affects cell growth, apoptosis and attachment in keratinocytes. Hafner C, Di Martino E, Pitt E, Stempfl T, Tomlinson D, Hartmann A, Landthaler M, Knowles M, Vogt T.Exp Cell Res. 2010 Jul 15;316(12):2008-16.

 

Mutant fibroblast growth factor receptor 3 induces intracellular signaling and cellular transformation in a cell type- and mutation-specific manner. di Martino E, L'Hôte CG, Kennedy W, Tomlinson DC, Knowles MA. Oncogene. 2009 Dec 3;28(48):4306-16.

 

Fibroblast growth factor receptor 1 promotes proliferation and survival via activation of the mitogen-activated protein kinase pathway in bladder cancer. Tomlinson DC, Lamont FR, Shnyder SD, Knowles MA. Cancer Res. 2009 Jun 1;69(11):4613-20.

 

Inactivation of the Rb pathway and overexpression of both isoforms of E2F3 are obligate events in bladder tumours with 6p22 amplification.  Hurst CD, Tomlinson DC, Williams SV, Platt FM, Knowles MA.  Oncogene. 2008 Apr 24;27(19):2716-27.

 

FGFR3 protein expression and its relationship to mutation status and prognostic variables in bladder cancer.  Tomlinson DC, Baldo O, Harnden P, Knowles MA.  J Pathol. 2007 Sep;213(1):91-8

 

Knockdown by shRNA identifies S249C mutant FGFR3 as a potential therapeutic target in bladder cancer.  Tomlinson DC, Hurst CD, Knowles MA.  Oncogene. 2007 Aug 30;26(40):5889-99.

 

Alternative splicing of fibroblast growth factor receptor 3 produces a secreted isoform that inhibits fibroblast growth factor-induced proliferation and is repressed in urothelial carcinoma cell lines. Tomlinson DC, L'Hote CG, Kennedy W, Pitt E, Knowles MA. Cancer Research 2005;65(22):10441-9.

 

FGFR3 and Ras gene mutations are mutually exclusive genetic events in urothelial cell carcinoma. Jebar AH, Hurst CD, Tomlinson DC, Johnston C, Taylor CF, Knowles MA. Oncogene 2005;24(33):5218-25.

 

Differential effects of transforming growth factor-beta1 on cellular proliferation in the developing prostate. Tomlinson DC, Freestone SH, Grace OC, Thomson AA.  Endocrinology. 2004 Sep;145(9):4292-300. Epub 2004 Jun 10.

 

Regulation of Fgf10 gene expression in the prostate: identification of transforming growth factor-beta1 and promoter elements. Tomlinson DC, Grindley JC, Thomson AA. Endocrinology. 2004 Apr;145(4):1988-95. Epub 2004 Jan 15.

 

Sonic hedgehog regulates prostatic growth and epithelial differentiation. Freestone SH, Marker P, Grace OC, Tomlinson DC, Cunha GR, Harnden P, Thomson AA. Dev Biol. 2003 Dec 15;264(2):352-62.